Journal: Cancer cell

Article Title: Binary pan-cancer classes with distinct vulnerabilities defined by pro- or anti-cancer YAP/TEAD activity

doi: 10.1016/j.ccell.2021.06.016

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: NCI-H2171 SCLC cells were cultured in DMEM:F12 media with 5% FBS, 0.005 mg/ml insulin, 0.01 mg/ml bovine transferrin (Thermo Fisher), 30 nM sodium selenite (Sigma), 10 nM hydrocortisone (Sigma), 10 nM β-estradiol (Sigma) and extra 2mM L-glutamine (for final conc. of 4.5 mM).

Techniques: Virus, Plasmid Preparation, Derivative Assay, Recombinant, Electron Microscopy, Staining, SYBR Green Assay, Reverse Transcription, Sample Prep, Microarray, Knockdown, Gene Expression, CRISPR, Software, Flow Cytometry

Journal: Molecular therapy : the journal of the American Society of Gene Therapy

Article Title: Efficient generation of functional hepatocytes from human embryonic stem cells and induced pluripotent stem cells by HNF4α transduction.

doi: 10.1038/mt.2011.234

Figure Lengend Snippet: Figure 2 Hepatic differentiation of human ESCs and iPSCs transduced with three factors. (a) The procedure for differentiation of human ESCs and iPSCs into hepatocytes via DE cells and hepatoblasts is presented schematically. The hESF-DIF medium was supplemented with 10 μg/ml human recombinant insulin, 5 μg/ml human apotransferrin, 10 μmol/l 2-mercaptoethanol, 10 μmol/l ethanolamine, 10 μmol/l sodium selenite, and 0.5 mg/ml fatty-acid-free BSA. The L15 medium was supplemented with 8.3% tryptose phosphate broth, 8.3% FBS, 10 μmol/l hydrocortisone 21- hemisuccinate, 1 μmol/l insulin, and 25 mmol/l NaHCO3. (b) Sequential morphological changes (day 0–20) of human ESCs (H9) differentiated into hepatocytes via DE cells and hepatoblasts are shown. Red arrow shows the cells that have double nuclei. (c) The morphology of primary human hepatocytes is shown. Bar represents 50 μm. BSA, bovine serum albumin; DE, definitive endoderm; ESC, embryonic stem cell; iPSC, induced pluripotent stem cell.

Article Snippet: The differentiation protocol for the induction of DE cells, hepatoblasts, and hepatocytes was based on our previous report with some modifications.7 Briefly, in mesendoderm differentiation, human ESCs and iPSCs were dissociated into single cells and cultured for 3 days on Matrigel (Becton, Dickinson and Company, Tokyo, Japan) in hESF-DIF medium (Cell Science & Technology Institute) supplemented with 10 g/ ml human recombinant insulin, 5 g/ml human apotransferrin, 10 mol/l 2-mercaptoethanol, 10 mol/l ethanolamine, 10 mol/l sodium selenite, 0.5 mg/ml BSA, and 100 ng/ml Activin A (R&D Systems, Minneapolis, MN).

Techniques: Transduction, Recombinant